Program

Registration

Welcome and Opening Remarks

Session 1: Top-Down Proteomics, Part I

Top-down proteomics and proteoforms – a versatile playground for analytical chemistry

Andreas Tholey (Christian-Albrechts-Universität Kiel)

Truncated Proteoforms in Top-Down Proteomics Studies

Philipp T Kaulich (Christian-Albrechts-Universität Kiel)

Image-Guided 3D Laser Sampling for Subsequent Deep Omics: Background, Methodology and Applications in Cancer Research

Jan Hahn (University Medical Center Hamburg-Eppendorf)

Via infrared-laser based sampling towards the original composition of proteoforms in tissues

Hartmut Schlüter (University Medical Center Hamburg-Eppendorf)

Coffee Break

Session 2: Proteoform Analysis with Bottom-Up Approach

Elucidation of regulatory mechanisms of protein kinases based on kinome profiling

Naoyuki Sugiyama (National Cerebral and Cardiovascular Center)

Defining proteoforms through post-translational modification proteomics

Kazuya Tsumagari (RIKEN)

Significance of Protein Terminus Excavation as a Trace of RNA Splicing, Translation and Proteolysis

Yasushi Ishihama (Kyoto University)

Short Break

Session 3: Lightning Talks by Japanese Student Participants

Large-scale advanced design and evaluation of kinase-specific artificial substrate peptides

Liang Junqi (Kyoto University)

Ultrahigh-speed, high-sensitivity and high-depth proteomics by capillary-flow LC/MS/MS

Ayana Tomioka (Kyoto University)

Break

Session 4: Proteofom Analysis in Clinical/Biological Studies

Patient-derived cell models for the elucidation of molecular mechanisms in tissue regeneration and lung barrier integrity

Sabine Fuchs (University Medical Center Schleswig-Holstein)

The protein levels of post-transcriptionally regulated genes determine stem cell fate into three germ layers

Mio Iwasaki (Kyoto University)

In Variatate Concordia: Can single-molecule DNA and RNA sequencing in stem cell systems inform proteoform mapping?

Franz-Josef Müller (University Hospital Schleswig-Holstein)

Break

Welcome Party

Registration

Session 5: Top-Down Proteomics, Part II

Characterization of proteoforms of intact proteins by one and two-dimensional CE-MS techniques

Christian Neusüß (Aalen University)

Computational methods in top-down proteomics to address challenges in proteoform analysis

Kyowon Jeong (University of Tübingen)

OpenMS - An open-source software suite for (top-down) proteomics

Oliver Kohlbacher (University of Tübingen)

Coffee Break

Session 6: Promising Technologies for Proteoform Analysis

Proteomics with cell-free synthesized peptides/proteins

Yoshihiro Shimizu (RIKEN)

Development of tapping-mode scanning probe electrospray ionization for mass spectrometry imaging of tissues and cells

Yoichi Otsuka (Osaka University)

Multi-dimensional vibrational circular dichroism as targeted for biological samples

Hisako Sato (Ehime University)

Lunch

Session 7: Top-Down/Bottom-Up Peptidomics

Biomedical applications of peptidomics, a subdivision of proteoform profiling

Kazuki Sasaki (Tochigi Cancer Center)

Ion mobility mass spectrometry for immunopeptidomics in cancer immunotherapy

Yuriko Minegishi (Japanese Foundation for Cancer Research)

Peptidomic analyses of plasma and tissues based on high-yield peptide extraction method

Yoshio Kodera (Kitasato University)

Coffee Break

Session 8: Structural and Single Molecule Analysis Techniques for Proteoform Analysis

Nanofluidic and Lab on a Chip devices for single molecule analysis

Irene Fernandez-Cuesta (University of Hamburg)

High-precision proteoform quantification reveals phosphorylation kinetics of AMP-activated protein kinase

Boris Krichel (Univ. Lübeck & Centre for Structural Systems Biology)

Connecting proteoforms to higher-order structure by combining native and top-down MS

Frederik Lermyte (Technical University of Darmstadt)

Break

Session 9: Gel-Based Proteoform Analysis

Gel-Based Human Proteoform Atlas: Mapping of Human Proteoforms using SDS Polyacrylamide Gel Electrophoresis

Nobuaki Takemori (Ehime University)

Development of Auto-2D: a fully automated two-dimensional electrophoresis system, and application for the top-down proteomics to identify proteoforms in tumor samples

Norie Araki (Kumamoto University)

Final Remarks